poca-SMLM.github.io

• Open a localization dataset (for instance simulation_1.csv in the folder data, check Section Opening localization files).

• Create a Delaunay triangulation (check Section on Delaunay triangulation)

• When rendering the Delaunay triangulation, please make sure that the Cull face is not selected. Culling is an OpenGL process that will not rendering some triangles depending on their orientation. When on, it may seem like some tetrahedra were not computed.

• Chnage the volume feature to logarithmic distribution and toggle off the rendering of the localizations (check the Voronoi use case for more information). Then apply a cutting distance, in this case 45 nm. This distance may be different depending on your data.

• If the thresholding is fine, create objects and toggle the Delaunay rendering off.

• Save the objects’ statistics.

You can find the corresponding macro here.

Back to main page